Publikationen der Arbeitsgruppe Prof. Dr. Unden

Genregulation durch Umweltsignale

Unden G., Guest J.R. (1985) Isolation and characterization of the FNR protein, the transcripitonal regulator of anaerobic electron transport in Escherichia coli. Eur. J. Biochem 146: 193-199

Unden G., Duchéne (1987) On the role of cyclic AMP and the FNR protein in E. coli growing anaerobically. Arch. Microbiol 147: 195-200

Unden G. (1988) Differential roles for menaquinone and demethylmenaquione in anaerobic electron transport of E. coli and their fnr-independent expression. Arch. Microbiol 150:499-503

Trageser M., Unden G. (1989) Role of cysteine residues and metal ions in the regulatory functioning of FNR, the transcriptional regulator of anaerobic respiration in E. coli. Mol. Microbiol 3(5): 593-599

Trageser M., Spiro S., Duchéne A., Kojro E., Fahrenholz F., Guest J.R., Unden G. (1990) Isolation of intact FNR protein (Mr 30 000) of E. coli. Mol. Microbiol 4(1): 21-27

Unden G., Trageser M., Duchéne A. (1990) Effect of positive redox potentials on the expression of anaerobic respiratory enzymes in E.coli. Mol. Microbiol 4(2): 315-319

Unden G., Trageser M. (1991) (Review) Oxygen regulated gene expression in Escherichia coli: control of anaerobic respiration by the FNR protein. Ant van Leeuwenhoek 59: 65-76

Engel P., Trageser M., Unden G. (1991) Reversible interconversion of the functional state of the gene regulator FNR from Escherichia coli in vivo by O2 and iron availability. Arch. Microbiol 156: 463-470

Niehaus F., Hantke K., Unden G. (1991) Iron content and FNR-dependent gene regulation in Escherichia coli. FEMS Microbiol Lett 84: 319-323

Schlüter A., Patschkowski T., Unden G., Priefer U. (1992) The Rhizobium leguminosarum FNRN Protein is functionally similar to Escherichia coli FNR and promotes heterologous oxygen-dependent activation of transcription. Mol. Microbiol 6(22): 3395-3404

Engel P., Krämer R., Unden G. (1992) Anaerobic fumarate transport in Escherichia coli by an fnr - dependent dicarboxylate uptake system which is different from aerobic dicarboxylate uptake. J. Bacteriol 174: 5533-5539

Lorenzen J.P., Kröger A., Unden G. (1993) Regulation of anaerobic respiratory pathways in Wolinella succinogenes by the presence of electron acceptors. Arch. Microbiol 159: 477-483

Unden G., Becker S., Bongaerts J., Schirawski J., Six S. (1994) (Review) Oxygen regulated gene expression in facultatively anaerobic bacteria. Ant van Leeuwenhoek. 66: 3-23

Six S., Andrews S.C., Unden G., Guest J.R. (1994) Escherichia coli possesses two homologous anaerobic C4-dicarboxylate membrane transporters (DcuA and DcuB) distinct from the aerobic dicarboxylate transport system (Dct). J. Bacteriol 176: 6470-6478

Schirawski J., Unden G. (1995) Anaerobic respiration of Bacillus macerans with fumarate, TMAO, nitrate and nitrite and regulation of the pathways by oxygen and nitrate. Arch. Microbiol, 163: 148-154

Bongaerts J., Zoske S., Weidner U., Unden G. (1995) Transcriptional regulation of the proton translocating NADH dehydrogenase genes (nuoA-N) of Escherichia coli by electron acceptors, electron donors and gene regulators. Mol. Microbiol, 16: 521-534

Unden G., Bongaerts J., Becker S., Holighaus G., Schirawski J., Six S. (1995) (Review) O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria. Arch Microbiol, 164: 81-90

Becker S., Holighaus G., Gabrielczyk T., Unden G. (1996) O2 as the regulatory signal for FNR-dependent gene regulation in Escherichia coli. J Bacteriol, 178: 4515-4521

Unden G., Bongaerts J. (1997) (Review) Alternative respiratory pathways of Escherichia coli: Energetics and transcriptional regulation in response to electron acceptors. Biochim. Biophys. Acta, 1320: 217-234

Unden G., Schirawski J. (1997) (Review) The O2 responsive transcriptional regulator FNR of Escherichia coli: The search for signals and reactions. Mol. Microbiol, 25: 205-210

Becker S., Vlad D., Schuster S., Pfeiffer P., Unden G. (1997) Regulatory O2 tensions for the synthesis of fermentation products in Escherichia coli and relation to aerobic respiration. Arch Microbiol, 168: 290-296

Unden G. (1998) Aerobic respiration and regulation of aerobic and anaerobic metabolism. In: H. G. Schlegel, J. Lengeler (eds.) Biology of the Prokaryotes, 262-278

Prohl C., Wackwitz B., Vlad D., Unden G. (1998) Functional citric acid cycle in an arcA mutant of Escherichia coli during growth with nitrate under anoxic conditions. Arch. Microbiol, 170: 1-7

Unden G. (1998) Transcriptional regulation and energetics of alternative respiratory pathways in facultatively anaerobic bacteria. Biochim Biophys Acta, 1365: 220-224

Arras T, Schirawski J, Unden G (1998) Availability of O2 as a substrate in the cytoplasm of bacteria under oxic and micro-oxic conditions. J. Bacteriol, 180: 2133-2136

Klinger A., Schirawski J., Glaser P., Unden G. (1998) The fnr gene of Bacillus licheniformis and the cysteine ligands of the C-terminal Teschuster. J. Bacteriol. 180: 3483-3485

Zientz E., Bongaerts J., Unden G. (1998) Fumarate regulation of gene expression in Escherichia coli by the Dcu SR (dcu SR genes) two-component regulatory system. J. Bacteriol. 180: 5421-5425

Schirawski J., Hankeln T, Unden G. (1998) Expression of the succinate dehydrogenase genes (sdh CAB) from the facultatively anaerobic Parnibacillus macerans during aerobic growth. Arch. Microbiol, 170: 304-308

Wackwitz B., Bongaerts J., Goodman S., Unden G. (1999) Growth phase dependent regulation of nouA-N expression of Escherichia coli by the Fis and IHF proteins: DNA binding and bioenergietic significance. Mol. Gen. Genet.

Tran Q. H., Arras T., Becker S., Holighaus G., Ohlberger G., Unden G. (2000) Role of glutathione in the formation of the active form of the oxygen sensor FNR ([4Fe-4S].FNR) and in the control of FNR function. Eur. J. Biochem. 267: 4817-4824

Biochemie des fakultativ anaeroben Stoffwechsels

Unden G., Hackenberg H., Kröger A. (1980) Isolation and functional aspects of the fumarate reductase involed in phosphorylative electron transport of Vibrio succinogenes. Biochim. Biophy.s Acta 591: 275-288

Unden G., Kröger A. (1980) An essential sulfhydryl group at the substrate site of the fumarate reductase of V. succinogenes. FEBS Letter 117: 323-326

Albrecht S.P.J., Unden G., Kröger A. (1981) Iron-sulphur clusters in fumarate reductase from V. succinogenes. Biochim. Biophys. Acta 661: 295-302

Unden G., Kröger A. (1981) The function of the subunits of the fumarate reductase complex of V. succinogenes. Eur. J. Biochem 120: 577-584

Unden G., Böcher R., Knecht J., Kröger A. (1982) Hydrogenase from Vibrio succinogenes, a nickel protein. FEBS Letters 145: 230-234

Unden G., Kröger A. (1982) Reconstitution in liposomes of the electron transport chein catalyzing fumarate reduction by formate. Biochim. Biophy.s Acta 682: 258-263

Unden G., Cole S.T. (1983) Sudies on the relatedness of bacterial fumarate reductases. FEMS Microbiol Letter 20: 181-185

Unden G., Albracht S.P.J., Kröger A. (1984) Redox potential and kinetic properties of fumarate reductase complex from Vibrio succinogenes. Biochim Biophys Acta 767: 460-469

Wissenbach U., Kröger A., Unden G. (1990) The specific functions of menaquinone and demethylmenaquinone in anaerobic respiration with fumarate, dimethylsulfoxide, trimethylamine N-oxide and nitrate by Escherichia coli. Arch. Microbiol. 154: 60-66

Kröger A., Unden G. (1985) The function of menaquinone in bacterial electron transport. In ?Coenzyme Q? (Ed. G. Lenaz), John Wiley & Sons Ltd. Chichester, pp. 285-300

Unden G., Kröger A. (1986) Reconstitution of a functional electron transport chein from isolated enzymes in liposomes. Methods in Enzymology, Vol 126: 38
7-399

Albracht S.P.J., Kröger A., van der Zwaan J.W., Unden G., Böcher R., Mell H., Fontijy R.D. (1987) Direct evidence for sulphur as aligand to nickel in hydrogenase: an EPR sutdy of the enzyme from Wolinella succinogenes enriched in 33S. Biochim. Biophys. Acta 874: 116-127

Lauterbach F., Körtner Ch., Tripier D., Unden G. (1987) Cloning and expression of the genes of two fumarate reductase subunits from Wolinella succinogenes. Eur. J. Biochem. 166: 447-452

Unden G. (1988) Differential roles for menaquinone and demethylmenaquinone in anaerobic electron transport of E. coli and their fnr-independent expression. Arch. Microbiol. 150: 499-503

Körtner C., Lauterbach F., Tripier D., Unden G., Kröger A. (1990) Wolinella succinogenes fumarate reductase contains a dihaem cytochrome b. Mol. Microbiol. 4(5): 855-860

Lauterbach F., Körtner C., Albracht S.P.J., Unden G., Kröger A. (1990) The fumarate reductase operon of Wolinella succinogenes: Sequence and expression of the frdA and frdB genes. Arch. Microbiol. 154: 386-393

Lemma E., Unden G., Kröger A. (1990) Menaquinone is an obligatory component of the chain catalyzing succinate respiration in Bacillus subtilis. Arch. Microbiol. 155: 62-67

Wissenbach U., Ternes D., Unden G. (1992) An Escherichia coli mutant containing only demethylmenaquinone, but no menaquinone: effects on fumarate, DMSO, TMAO and nitrate respiration. Arch. Microbiol. 158: 68-73

Engel P., Krämer R., Unden G. (1992) Anaerobic fumarate transport in Escherichia coli by an fnr - dependent dicarboxylate uptake system which is different from aerobic dicarboxylate uptake. J. Bacteriol. 174: 5533-5539

Lorenzen J.P., Kröger A., Unden G. (1993) Regulation of anaerobic respiratory pathways in Wolinella succinogenes by the presence of electron acceptors. Arch. Microbiol 159: 477-483

Wissenbach U., Keck B., Unden G. (1993) Physical map location of the new artPIQMJ genes of Escherichia coli, encoding a periplasmic arginine transport system. J. Bacteriol. 175: 3687

Engel P., Krämer R., Unden G. (1994) Transport of C4-dicarboxylates by anaerobically grown Escherichia coli: Energetics and mechanism of exchange, uptake and efflux. Eur. J. Biochem. 222: 605-614

Lorenzen J., Steinwachs S., Unden G. (1994) DMSO respiration by the anaerobic rumen bacterium Wolinella succinogenes. Arch. Microbiol. 162: 277-281

Schirawski J., Unden G. (1995) Anaerobic respiration of Bacillus macerans with fumarate, TMAO, nitrate and nitrite and regulation of the pathways by oxygen and nitrate. Arch. Microbiol. 163: 148-154

Wissenbach U., Six S., Bongaerts J., Steinwachs S., Unden G. (1995) A third periplasmic transport system for L-arginine in Escherichia coli: Molecular characterization of the artPIQMJ genes, arginine binding and transport. Mol. Microbiol, 17: 675-686

Zientz E., Six S., Unden G. (1996) Identification of a third secondary carrier (DcuC) for anaerobic C4-dicarboxylate transport in Escherichia coli: Role of the three Dcu carriers in uptake and exchange. J. Bacteriol. 178: 7241-7247

Tran Q.H., Bongaerts J., Vlad D., Unden G .(1997) Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in NADH-fumarate respiration and bioenergetic implications. Eur. J. Biochem. 244: 155-160

Unden G., Bongaerts J. (1997) (Review) Alternative respiratory pathways of Escherichia coli: Energetics and transcriptional regulation in response to electron acceptors. Biochim. Biophys. Acta 1320: 217-234

Tran Q.H., Unden G. (1998) Changes in the proton potential and the cellular energetics of Escherichia coli during growth by aerobic or anaerobic respiration or by fermentation. Eur. J. Biochem, 251: 538-543

Unden G. (1998) Aerobic respiration and regulation of aerobic and anaerobic metabolism. In: H. G. Schlegel, J. Lengeler (eds.) Biology of the Prokaryotes, 262-278.

Unden G. (1998) Transcriptional regulation and energetics of alternative respiratory pathways in facultatively anaerobic bacteria. Biochim. Biophys. Acta, im Druck

Schirawski J., Hankeln T., Unden G. (1998) Expression of the succinate dehydrogenase genes (sdhCAB) from the facultatively anaerobic Paenibacillus macerans during aerobic growth. Arch. Microbiol 170: 304-308

Schirawski J., Unden G. (1998) Menaquinone dependent succinate dehydrogenase of bacteria catalyzes reversed electron transport driven by the proton potential. Eur. J. Biochem, 257: 210-215

Unden G. (1999) Aerobic respirations and regulation of aerobic and anaerobic metabolism. In: H. G. Schlegel, J. Lengeler (eds.). Biology of the Prokaryotes, 261-277

Janausch I.G., Unden G. (1999) The dcud (former yhcL) gene of Escherichia coli as a member of the DcuC family of a C4-dicarboxylate carries: lack of evident expression. Arch. Microbiol. 172: 219-226